Fig. 2. Atp6-CSTC Om45-GFP cells are temperature sensitive and display hydrogen peroxide and calcium ion sensitivity suppressed by Tim11 deletion. A) Growth phenotypes. Overnight precultures were serially diluted 10-fold, spotted on plates and incubated at the indicated temperatures for 2-4 days. Representative plates are shown. B) Stability of mtDNA. Cells were grown at permissive temperature in YPGalA medium to an OD of 4. Approximately 100 cells were spread on YPGA plates and grown, and the percentage of petite colonies was counted. Experiments were performed in at least four replicates.